TYPE OF STROMAL COLLAGEN FIBERS IN THE ORAL SQUAMOUS CELL CARCINOMA… A HISTOCHEMICAL STUDY USING PICROSIRIUS RED STAIN
Objective: Determination of the type of stromal collagen fibers in the Oral Squamous Cell Carcinoma.
Materials and Methods: Biopsies were taken from 71 patients of oral squamous cell carcinoma. Slides were stained with Picrosirius red (PSR) stain. All the samples were evaluated for the type of collagen fibers by examining under light microscope using polarizing lens.
Results: Type III collagen was the predominant type of collagen seen in the poorly differentiated oral squamous cell carcinoma cases, whereas type I collagen fibers were sparse. The percentage of type I collagen was highest in well differentiated oral squamous cell carcinoma cases and type III collagen fibers were infrequent. While the percentage of type III collagen fibers was more than type I collagen fibers in the moderately differentiated oral squamous cell carcinoma cases. In the present study, this variation in types of collagen fibers in the three histological grades of oral squamous cell carcinoma was found to be statistically significant (p= 0.08).
Conclusion: In the light of the results of the present study it is concluded the collagen profiling can be effectively used as a prognostic marker in oral squamous cell carcinoma cases. Abundance of type III collagen fibers in tumor stroma seems to be a poor prognostic indicator.