Effect of Moringa Oleifera Leaves on Bisphenol-A Induced Histological Changes of Hepatocytes in  Albino Rats

Attya Zaheer; Shakeela  Nazir; Nazia  Sidiqque; Fatima  Qamar; Ashiq  Hussain; Mushtaq  Ahmed

doi:10.51642/ppmj.v30i01.255

Attya Zaheer Post Graduae Medical Instituite, Lahore
Shakeela Nazir Rashid Latif Medical College
Nazia Sidiqque Al-Aleem Medical College
Fatima Qamar Rashid Latif Medical College
Ashiq Hussain Rashid Latif Medical College
Mushtaq Ahmed Amna Inayat Medical college

DOI: https://doi.org/10.51642/ppmj.v30i01.255

Keywords: BPA: Bisphenol-A, MoLE: Moringa Oleifera Leave extract.

Abstract

Objectives: To observe the effects of Moringa Oleifera leaves extract on BPA induced changes (Diameter of hepatocytes and vacuolization) in liver of rats.

Study Design: Experimental study, conducted at Post Graduate Medical Institute.

Methodology: This study was performed on 32 adult rats for seven weeks. They were divided into 4 equal groups A, B, C and D. Group A was control received corn oil only. Group B, received BPA only 50mg/kg/bw. Group C and D received BPA 50mg/kg along with MoLE 250mg and 500mg. Liver was removed and was fixed in 10% formalin. To observe the effect of BPA and MoLE, slides were prepared for histological examination. For Hepatocytes, diameter and vacuolization was observed. The statistical analysis of results was done by using SPSS 21.

Result: In group B, vacuolization (87.5% of animals) and statistical significant increase in mean diameter (19.7±1.3) of hepatocytes was seen. Presence of vacuoles has presumably lead to an increase in diameter . However in Groups C 50% of animals showed vacuolization and mean mean hepatocyte diameter was 17.0±1.1. In Group D 25% of animals showed vacuolization and mean mean hepatocyte diameter decreased to14.6±1 after administration of MoLE.