HISTOLOGICAL EFFECTS OF CARBONATED DRINKS ON ORAL SUBMUCOSAL HEALING IN ALBINO WISTA RATS

Abstract

Objective: In this study the effects of carbonated drinks on the healing process of oral submucosa of albino wistar rats were evaluated.

Design: This study comprised of 24 adult albino wistar rats which were randomly assigned to a experimental group 1 and an experimental group 2. A circular wound of 3.0 mm was created on the buccal mucosa of each albino wistar rat at day 0. Animals in control group were fed with chow pellet and water while those in experimental group were fed with chow pellet and a commercially available carbonated drink instead of water. 6 animals from each group were sacrificed by decapitation under deep anesthesia at day 7 and 21. The tissue dissected from buccal area was serially sectioned and stained with Haematoxylin & Eosin stains. Wound site was histologically assessed for differences in the healing pattern of submucosa; inflammatory cell count and neovascularization between two groups.

Results: There was a marked difference in the healing pattern between the two groups. Animals in group-1 showed a normal healing pattern with formation of a fibrous connective tissue at the end of day-21. In the group-2, there is altered healing phenomenon at the end of the experiment with a subsequent delayed inflammatory reaction at day-21

Conclusion: These findings suggest that consumption of carbonated drinks can disrupt oral wound healing. The contents in carbonated drinks have a proinflammatory action on the soft tissue.